Molecular-scale mapping of the Pseudomonas aeruginosa biofilm matrix

Research Focus of the Reichhardt Lab: The overarching goal of the Reichhardt Lab is to discover the fundamental biophysical principles of biofilm assembly. Within biofilms, aggregates of microbes are encased in a mesh-like, biopolymer-rich matrix that promotes microbial cell-cell interactions and contribute to biofilms causing difficult-to-treat chronic infections. Despite the importance of the biofilm matrix, we still do not understand how individual matrix components are assembled into a functional architecture. Since this problem spans several scales—multicellular to atomic—new multidisciplinary approaches are required. Therefore, we are developing approaches that integrate microbiological methods with physical chemistry tools including microscopy and solid-state nuclear magnetic resonance (NMR). Overall, our research program aims to provide critical understanding of biofilm assembly, with broader impact on bacterial physiology, materials science, and treatment for chronic infections.